Human USP7 (+/-) Knockout Cell Line-HCT116

Name: Human USP7 (+/-) Knockout Cell Line-HCT116
SKU: CSC-RT0062
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0062

Target Gene : USP7 Host Cell : HCT116

Size : >1x10⁶ cells/vial Validation : Sequencing

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT0062
Description	HCT116 -USP7 (+/-) is a cell line with a heterozygous knockout of human USP7
Target Gene	USP7
Host Cell	HCT116
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	USP7 ubiquitin specific peptidase 7 (herpes virus-associated) [ Homo sapiens ]
Gene Symbol	USP7
Synonyms	TEF1; HAUSP
Gene Description	ubiquitin specific peptidase 7 (herpes virus-associated)
Gene ID	7874
Uni Prot ID	Q6U8A4
m RNA Refseq	NM_003470.2
Protein Refseq	NP_003461.2
Chromosome Location	16p13.3
Function	cysteine-type endopeptidase activity; p53 binding; protein C-terminus binding; protein binding; protein homodimerization activity; transcription factor binding; ubiquitin protein ligase binding; ubiquitin thiolesterase activity; ubiquitin thiolesterase activity; ubiquitin-specific protease activity; ubiquitin-specific protease activity;
Pathway	Epstein-Barr virus infection, organism-specific biosystem; Epstein-Barr virus infection, conserved biosystem; FoxO family signaling, organism-specific biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; Viral carcinogenesis, organism-specific biosystem; Viral carcinogenesis, conserved biosystem;
MIM	602519

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *