Panoply™ Human USP7 Knockdown Stable Cell Line

Name: Panoply™ Human USP7 Knockdown Stable Cell Line
SKU: CSC-DC017200
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-DC017200

Host Cell : HEK293 (Hela and other cell types are also available) Validation : Real-Time RCR

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Cell Line Information

Safety and Packaging

Gene Information

Cat. No.	CSC-DC017200
Description	Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene	USP7
Host Cell	HEK293 (Hela and other cell types are also available)
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid Nitrogen
Shipping	Dry Ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	USP7 ubiquitin specific peptidase 7 (herpes virus-associated) [ Homo sapiens ]
Gene Symbol	USP7
Synonyms	TEF1; HAUSP
Gene Description	ubiquitin specific peptidase 7 (herpes virus-associated)
Gene ID	7874
Uni Prot ID	Q6U8A4
m RNA Refseq	NM_003470.2
Protein Refseq	NP_003461.2
Chromosome Location	16p13.3
Function	cysteine-type endopeptidase activity; p53 binding; protein C-terminus binding; protein binding; protein homodimerization activity; transcription factor binding; ubiquitin protein ligase binding; ubiquitin thiolesterase activity; ubiquitin thiolesterase activity; ubiquitin-specific protease activity; ubiquitin-specific protease activity;
Pathway	Epstein-Barr virus infection, organism-specific biosystem; Epstein-Barr virus infection, conserved biosystem; FoxO family signaling, organism-specific biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; Viral carcinogenesis, organism-specific biosystem; Viral carcinogenesis, conserved biosystem;
MIM	602519

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Q & A

Customer Reviews

Customer Q&As

How do two compounds, FT671 and FT827, inhibit?

A: Both compounds are able to target USP7 to automatically inhibit dynamic pocketing near the catalytic centre of the Apo protein white structure.

What is the response of FT671 in cells?

A: Consistent with the intracellular involvement of USP7, FT671 destabilizes USP7-responsive substrates, including MDM2, increases p53 levels, leads to transcription of p53 target genes, induces the tumor suppressor gene p21, and ultimately inhibits tumor creation in mice.

Physiological process of USP7?

A: One enzyme in the regulation of ubiquitination, ubiquitin-specific protease 7 (USP7), catalyzes the removal of the ubiquitin chain from the substrate. Usually ubiquitin labelling of a protein means that it is likely to be degraded

What is the impact of the deubiquitination of USP7?

A: The deubiquitination of USP7 facilitates protein stabilization. In the regulation of histone methylation, PHD zinc finger protein 8 (PHF8) is a histone lysine demethylase that demethylates the H3 subunit of histones. PHF8 is already known to be associated with the development of many diseases.

In what tissues is USP7 overexpressed?

A: USP7 is overexpressed in a variety of cancers such as prostate, bladder, colon, liver and lung cancers.

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