Human TNF HEK293 Cell Lysate

Name: Human TNF HEK293 Cell Lysate
SKU: CLOE-1311
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1311 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1311
Introduction	This cell lysate over-expresses the human TNF (NP_000585.2) (Val77-Leu233) fused with the Fc region of human IgG1 at the N-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	TNF
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	TNF tumor necrosis factor [ Homo sapiens ]
Gene Symbol	TNF
Synonyms	TNF; tumor necrosis factor; TNFA, tumor necrosis factor (TNF superfamily, member 2); DIF; TNF superfamily; member 2; TNF alpha; TNFSF2; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; TNF superfamily, member 2; tumor necrosis factor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; TNFA; TNF-alpha;
Gene ID	7124
Uni Prot ID	P01375
m RNA Refseq	BC028148
Chromosome Location	6p21.3
Function	cytokine activity; identical protein binding; protease binding; protein binding; transcription regulatory region DNA binding; tumor necrosis factor receptor binding;
Pathway	Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem;
MIM	191160

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