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Human ST6GAL1 lentiviral particles

For research use only. Not intended for any clinical use.

Cat. No. :   LV00484Z

Storage :   -80℃ Shipping :   Frozen on dry ice

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Virus Particles Information

Quality Control

Gene Information

Cat. No. LV00484Z
Description Human ST6GAL1 ORF cDNA is cloned into the lentiviral expression vector for maximal stable delivery. The ready-to-use lentiviruses accelerate experiments investigating exogenous gene overexpression phenotypes and downstream molecular impacts.
Product Type Lentiviral particle
Gene ST6GAL1
Species Human
Titer Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc.
Size Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Summary Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.
Gene Name ST6GAL1 ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 [ Homo sapiens ]
Gene Symbol ST6GAL1
Synonyms ST6N; SIAT1; ST6GalI
Gene Description ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
GeneID 6480
Uni ProtID P15907
mRNA Refseq NM_003032.2
Protein Refseq NP_003023.1
Chromosome Location 3q27-q28
Function beta-galactoside alpha-2,6-sialyltransferase activity; sialyltransferase activity;
Pathway Asparagine N-linked glycosylation, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; N-Glycan antennae elongation, organism-specific biosystem; N-Glycan biosynthesis, organism-specific biosystem; N-Glycan biosynthesis, conserved biosystem; N-glycan antennae elongation in the medial/trans-Golgi, organism-specific biosystem; N-glycan biosynthesis, complex type, organism-specific biosystem;
MIM 109675
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