Human SPP1 HEK293 Cell Lysate

Name: Human SPP1 HEK293 Cell Lysate
SKU: CLOE-1486
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1486 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1486
Introduction	This cell lysate over-expresses C-terminal polyhistidine tagged human SPP1 (NP_001035147.1) (Met 1-Asn 314). Purification of target protein (s) from this cell lysate has been verified.
Target Gene	SPP1
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	SPP1 secreted phosphoprotein 1 [ Homo sapiens ]
Gene Symbol	SPP1
Synonyms	OPN; BNSP; BSPI; ETA-1
Gene Description	secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
Gene ID	6696
Uni Prot ID	P10451
m RNA Refseq	NM_001040060.1
Protein Refseq	NP_001035149.1
Chromosome Location	4q22.1
Function	cytokine activity; extracellular matrix binding;
Pathway	Direct p53 effectors, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; Endochondral Ossification, organism-specific biosystem; FGF signaling pathway, organism-specific biosystem; Focal Adhesion, organism-specific biosystem; Focal adhesion, organism-specific biosystem;
MIM	166490

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