Human SLC11A2 Knockout Cell Line-caco2

Name: Human SLC11A2 Knockout Cell Line-caco2
SKU: CSC-RT2586
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2586

Target Gene : SLC11A2 Host Cell : Caco-2

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2586
Description	This cell is a stable cell line with a homozygous knockout of human SLC11A2 using CRISPR/Cas9.
Target Gene	SLC11A2
Host Cell	Caco-2
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing
Storage	Liquid nirtogen

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SLC11A2 solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 [ Homo sapiens ]
Gene Symbol	SLC11A2
Synonyms	DCT1; DMT1; NRAMP2
GeneID	4891
Uni ProtID	P49281
mRNA Refseq	NM_000617.2
Protein Refseq	NP_000608.1
Chromosome Location	12q13
Function	cadmium ion binding; cadmium ion transmembrane transporter activity; NOT calcium ion transmembrane transporter activity; NOT chromium ion transmembrane transporter activity; cobalt ion binding; cobalt ion transmembrane transporter activity; cobalt ion transmembrane transporter activity; copper ion binding; copper ion transmembrane transporter activity; ferrous iron transmembrane transporter activity; hydrogen ion transmembrane transporter activity; inorganic cation transmembrane transporter activity; iron ion binding; lead ion transmembrane transporter activity; manganese ion binding; manganese ion transmembrane transporter activity; nickel cation binding; nickel cation transmembrane transporter activity; nickel cation transmembrane transporter activity; protein binding; solute:hydrogen symporter activity; vanadium ion transmembrane transporter activity; zinc ion binding; zinc ion transmembrane transporter activity; NOT zinc ion transmembrane transporter activity;
Pathway	HIF-2-alpha transcription factor network, organism-specific biosystem; Iron uptake and transport, organism-specific biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Metal ion SLC transporters, organism-specific biosystem; Mineral absorption, organism-specific biosystem; Mineral absorption, conserved biosystem;
MIM	600523

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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