Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT0043
Target Gene : PRKDC Host Cell : HCT116
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT0043 |
| Description | HCT116-PRKDC (+/-) is a cell line with a heterozygous knockout of human PRKDC |
| Target Gene | PRKDC |
| Host Cell | HCT116 |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | PRKDC protein kinase, DNA-activated, catalytic polypeptide [ Homo sapiens ] |
| Gene Symbol | PRKDC |
| Synonyms | HYRC; p350; DNAPK; DNPK1; HYRC1; XRCC7; DNA-PKcs |
| Gene Description | protein kinase, DNA-activated, catalytic polypeptide |
| Gene ID | 5591 |
| Uni Prot ID | P78527 |
| m RNA Refseq | NM_006904.6 |
| Protein Refseq | NP_008835.5 |
| Chromosome Location | 8q11 |
| Pathway | BARD1 signaling events, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; Class I PI3K signaling events mediated by Akt, organism-specific biosystem; Coregulation of Androgen receptor activity, organism-specific biosystem; DNA Repair, organism-specific biosystem; |
| MIM | 600899 |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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I will definitely consider this company first when I have future requests for gene knockout experiments, and the products I ordered this time have yielded impressive results
The cells were not in good condition when I did the experiment, I thought my transfection was going to fail, but it worked and the results were good, this reagent is still great
This reagent has no cumbersome transfection steps and is highly efficient, one of the major factors in the success of my experiment
The major difference between this reagent and other companies is that he transfects very well on a very cheap basis, and cheap is still good.
For the same price, this brand is considered to be more reliable, I see that many authors of articles use this brand of cell lines, cheap and good.
For my article on this genetic area, I went through several articles and found that several people have used this brand, so I guess there must be a reason why I prefer the products that people recommend.
After validating the cell lines for the first time, I felt that my results were not quite right and there was no trend, so I discussed with the after-sales engineer the countermeasure and changed the measurement, and there was a trend.
After the use of this cell line, the experiment progressed quite quickly and saved a lot of effort.
This knockout cell line knocked out the gene relatively cleanly, I used western blot and the knocked out group was indeed expression free and the build was very successful.
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