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Human PPARD Knockout Cell Line-HCT116

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0058

Target Gene :   PPARD Host Cell :   HCT116

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0058
Description HCT116 -PPARD (-/-) is a cell line with a homozygous knockout of human PPARD
Target Gene PPARD
Host Cell HCT116
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name PPARD peroxisome proliferator-activated receptor delta [ Homo sapiens ]
Gene Symbol PPARD
Synonyms PPARD; peroxisome proliferator-activated receptor delta; peroxisome proliferative activated receptor, delta; FAAR; NR1C2; NUC1; NUCII; PPAR-beta; PPAR-delta; nuclear hormone receptor 1; nuclear receptor subfamily 1 group C member 2; peroxisome proliferator-activated receptor beta; peroxisome proliferator-activated nuclear receptor beta/delta variant 2; NUCI; PPARB; MGC3931;
GeneID 5467
Uni ProtID Q03181
mRNA Refseq BC002715
Chromosome Location 6p21.2
Function DNA binding; drug binding; fatty acid binding; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activi
Pathway Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adipogenesis, organism-specific biosystem; Energy Metabolism, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Nuclear Receptor transcription pathway, organism-specific biosystem;
MIM 600409
Quick Inquiry

Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Recommend

I can't recommend these PPARD knockout cell line enough! They're easy to use, highly effective, and have greatly improved my experimental results.

United States

Revolutionized, and efficient

These PPARD knockout cell line have revolutionized the way I conduct experiments. They're highly efficient and have saved me time and money.

United States

Accurate and reliable

This brand's PPARD knockout cell line are a game-changer! They've made my experiments more accurate and reliable. Thank you!

United States

Effective

I'm so impressed with this brand's PPARD knockout cell line. They're highly effective and have helped me achieve my research goals with ease.

United States

Good results

These PPARD knockout cell line are amazing! They've helped me achieve more accurate and consistent experimental results.

United States

Reasonable

I can't imagine conducting experiments without this brand's PPARD knockout cell line. They're reliable, affordable, and easy to use.

United States

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