Human MIF HEK293 Cell Lysate

Name: Human MIF HEK293 Cell Lysate
SKU: CLOE-1580
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1580 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1580
Introduction	This cell lysate over-expresses the human MIF (P14174) (Pro 2-Ala 115) fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	MIF
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	MIF macrophage migration inhibitory factor (glycosylation-inhibiting factor) [ Homo sapiens ]
Gene Symbol	MIF
Synonyms	GIF; GLIF; MMIF
Gene Description	macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Gene ID	4282
Uni Prot ID	I4AY87
m RNA Refseq	NM_002415.1
Protein Refseq	NP_002406.1
Chromosome Location	22q11.23
Function	cell surface binding; chemoattractant activity; cytokine activity; cytokine receptor binding; dopachrome isomerase activity; phenylpyruvate tautomerase activity; protein binding; receptor binding;
Pathway	Adipogenesis, organism-specific biosystem; Phenylalanine metabolism, organism-specific biosystem; Phenylalanine metabolism, conserved biosystem; Tyrosine metabolism, organism-specific biosystem; Tyrosine metabolism, conserved biosystem;
MIM	153620

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