Human LEPR(His) HEK293 Cell Lysate

Name: Human LEPR(His) HEK293 Cell Lysate
SKU: CLOE-1527
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1527 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1527
Introduction	This cell lysate over-expresses C-terminal polyhistidine tagged human LEPR (NP_002294.2) (Met 1-Asp 839). Purification of target protein (s) from this cell lysate has been verified.
Target Gene	LEPR
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	LEPR leptin receptor [ Homo sapiens ]
Gene Symbol	LEPR
Synonyms	OBR; OB-R; CD295; LEP-R
Gene ID	3953
Uni Prot ID	P48357
m RNA Refseq	NM_001003679.3
Protein Refseq	NP_001003679.1
Chromosome Location	1p31
Function	cytokine receptor activity; protein binding; transmembrane signaling receptor activity;
Pathway	AMPK signaling, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem;
MIM	601007

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