Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-1697 Host Cell : HEK293
| Cat. No. | CLOE-1697 |
| Introduction | This cell lysate over-expresses the extracellular domain of human L1CAM (NP_000416.1) (Met 1-Glu 1120) fused with a polyhistidine tag at the C-terminus. Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | L1CAM |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | L1CAM L1 cell adhesion molecule [ Homo sapiens ] |
| Gene Symbol | L1CAM |
| Synonyms | S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1 |
| Gene ID | 3897 |
| Uni Prot ID | P32004 |
| m RNA Refseq | NM_000425.3 |
| Protein Refseq | NP_000416.1 |
| Chromosome Location | Xq28 |
| Pathway | Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Basigin interactions, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; |
| MIM | 308840 |
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