Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-1775 Host Cell : HEK293
| Cat. No. | CLOE-1775 |
| Introduction | This cell lysate over-expresses the human IL12B (NP_002178.2) (Met 1-Ser 328) fused with the Fc region of human IgG1 at the C-terminus. Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | IL12B |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens ] |
| Gene Symbol | IL12B |
| Synonyms | CLMF; NKSF; CLMF2; NKSF2; IL-12B |
| Gene Description | interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) |
| GeneID | 3593 |
| Uni ProtID | P29460 |
| mRNA Refseq | NM_002187.2 |
| Protein Refseq | NP_002178.2 |
| Chromosome Location | 5q31.1-q33.1 |
| Function | contributes_to cytokine activity; cytokine receptor activity; contributes_to growth factor activity; identical protein binding; interleukin-12 alpha subunit binding; interleukin-12 receptor binding; contributes_to interleukin-12 receptor binding; contributes_to interleukin-23 receptor binding; protein binding; protein heterodimerization activity; protein homodimerization activity; |
| Pathway | African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; |
| MIM | 161561 |
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