Human GFP-NFkBp65/Hyg Reporter Stable Cell Line-CHO

Name: Human GFP-NFkBp65/Hyg Reporter Stable Cell Line-CHO
SKU: CSC-RR0038
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0038

Host Cell : CHO Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RR0038
Background	NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Target Gene	RELA
Abbr	CHO-HuGFP-NFkBp65/Hyg
Host Cell	CHO
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	RELA v-rel reticuloendotheliosis viral oncogene homolog A (avian) [ Homo sapiens ]
Gene Symbol	RELA
Synonyms	p65; NFKB3
Gene Description	v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65 (avian)
Gene ID	5970
Uni Prot ID	Q04206
m RNA Refseq	NM_001145138.1
Protein Refseq	NP_001138610.1
Chromosome Location	11q13
Function	DNA binding; NF-kappaB binding; RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; activating transcription factor binding; chromatin binding; identical protein binding; identical protein binding; phosphate ion binding; protein N-terminus binding; protein binding; protein kinase binding; repressing transcription factor binding; sequence-specific DNA binding transcription factor activity; transcription factor binding; transcription regulatory region DNA binding; ubiquitin protein ligase binding;
Pathway	Activated TLR4 signalling, organism-specific biosystem; Activation of NF-kappaB in B Cells, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem;
MIM	164014

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