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Human DYRK4 adenoviral particles

Human DYRK4 adenoviral particles

Cat.No. :  AD00158Z

Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL

Storage:  -80℃ Shipping:  Frozen on dry ice

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Adenovirus Particle Information

Quality Control

Cat. No. AD00158Z
Target Gene DYRK4
Product Type Adenoviral particle
Insert DYRK4
Titer Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc.
Size Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots.
Endotoxin Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance.
Sterility Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination.
Ad5 E1 Detection All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination.
RCA Assays Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources.
PFU Titering All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells.
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The Dual Specificity Tyrosine Phosphorylation Regulated Kinase 4 (DYRK4) gene encodes a member of the DYRK protein kinase family, which plays a critical role in processes such as cell proliferation, differentiation, and apoptosis. DYRK4 is characterized by its ability to phosphorylate both serine/threonine and tyrosine residues, which makes it a versatile regulator in signaling pathways. This kinase has been implicated in a variety of physiological and pathological states, including neurodevelopment, cancer progression, and metabolic regulation. Studies have shown that DYRK4 may affect cell cycle regulation and stress responses, making it a potential target for therapeutic intervention.

Human DYRK4 Adenoviral Particles are genetically engineered viral vectors designed to deliver the DYRK4 gene to target cells for functional studies or therapeutic purposes. These particles are derived from a replication-defective adenovirus, which ensures efficient transduction while minimizing the risks associated with viral replication. This adenoviral system has high transduction efficiencies in a variety of cell types, including dividing and non-dividing cells, making it a valuable tool for gene overexpression experiments. Human DYRK4 adenoviral particles enable researchers to study the role of DYRK4 in cellular pathways, disease models, or drug screening assays. In addition, these particles can be used in gene therapy studies to modulate the expression of DYRK4 in specific tissues.
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Customer Reviews
Excellent Customer Support

Not only is the product outstanding, but Creative Biogene’s team also provided detailed protocols and quick responses to our technical questions. A+ service!

Germany

05/12/2023

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