Human DICER1 Knockout Cell Line-DLD-1

Name: Human DICER1 Knockout Cell Line-DLD-1
SKU: CSC-RT0025
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0025

Target Gene : DICER1 Host Cell : DLD-1

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT0025
Description	DLD-1-DICER1 (-/-) is a cell line with a homozygous knockout of human DICER1
Target Gene	DICER1
Host Cell	DLD-1
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	DICER1 dicer 1, ribonuclease type III [ Homo sapiens ]
Gene Symbol	DICER1
Synonyms	DCR1; MNG1; Dicer; HERNA
Gene Description	Dicer1, Dcr-1 homolog (Drosophila)
Gene ID	23405
Uni Prot ID	Q9UPY3
m RNA Refseq	NM_030621.3
Protein Refseq	NP_085124.2
Chromosome Location	14q32.13
Function	ATP binding; ATP-dependent helicase activity; double-stranded RNA binding; metal ion binding; miRNA binding; protein binding; ribonuclease III activity;
Pathway	Gene Expression, organism-specific biosystem; MicroRNA (miRNA) Biogenesis, organism-specific biosystem; Regulatory RNA pathways, organism-specific biosystem; Small Interfering RNA (siRNA) Biogenesis, organism-specific biosystem; mRNA processing, organism-specific biosystem;
MIM	606241

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Good transfection results

This transfection reagent works very well, at least better than the one I used before, which was extremely unstable, like metaphysics

United States

2023-03-03

Good results

I used this reagent for one month and finished the experiment, the results are still stable and the transfection results are very good.

United States

2023-03-01

Stable results

If you follow the transfection steps for your experiments, you should reap good results, and the transfection reagent I constructed works just fine that way.

United States

2023-01-15

Trending in the right direction

The transfection results were very definitive and the results of the flow-through I did with the western blot were both very stable and the trends were consistent and correct.

United States

2023-03-16

Available results

I couldn't get results from my previous experiments, I verified them with western blot and I always suspected that my experimental technique was not up to scratch, in fact I think it was the reagents.

United States

2023-03-14

Costly and trustworthy

This cell line is, in all fairness, one of the best in its price range and the results are very trustworthy.

United States

2020-01-12

Efficient

The transfection was very successful and I summarized why, I found that this cell line was really different from the one I used before and very efficient.

United States

2021-04-11

Satisfied results

I have used this cell line a lot and have used some functional test consumables before and have been quite satisfied with the results.

United States

2021-11-21

Regular use

I am a regular user of this cell line company and I trusted this brand for all the genetic products I used for my last project.

United States

2022-01-31

So many advantages

This cell line is highly efficient and time-saving in transfection.Inexpensive, with many advantages in one.

United States

2022-08-05

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Human DICER1 Knockout Cell Line-DLD-1

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Q & A

Customer Reviews

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Good transfection results

Good results

Stable results

Trending in the right direction

Available results

Costly and trustworthy

Efficient

Satisfied results

Regular use

So many advantages

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