Human DCN(His) HEK293 Cell Lysate

Name: Human DCN(His) HEK293 Cell Lysate
SKU: CLOE-1636
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1636 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1636
Introduction	This cell lysate over-expresses C-terminal polyhistidine tagged human DCN (NP_001911.1) (Met 1-Lys 359). Purification of target protein (s) from this cell lysate has been verified.
Target Gene	DCN
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	DCN decorin [ Homo sapiens ]
Gene Symbol	DCN
Synonyms	CSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
Gene Description	decorin
Gene ID	1634
Uni Prot ID	P07585
m RNA Refseq	NM_133505.2
Protein Refseq	NP_598012.1
Chromosome Location	12q21.33
Function	collagen binding; extracellular matrix binding; glycosaminoglycan binding; protein N-terminus binding;
Pathway	A tetrasaccharide linker sequence is required for GAG synthesis, organism-specific biosystem; CS/DS degradation, organism-specific biosystem; Chondroitin sulfate biosynthesis, organism-specific biosystem; Chondroitin sulfate/dermatan sulfate metabolism, organism-specific biosystem; Dermatan sulfate biosynthesis, organism-specific biosystem; Disease, organism-specific biosystem; Glycosaminoglycan metabolism, organism-specific biosystem;
MIM	125255

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