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Human CSF1 lentiviral particles

For research use only. Not intended for any clinical use.

Cat. No. :   LV00245Z

Storage :   -80℃ Shipping :   Frozen on dry ice

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Virus Particles Information

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Gene Information

Cat. No. LV00245Z
Description Human CSF1 ORF cDNA is cloned into the lentiviral expression vector for maximal stable delivery. The ready-to-use lentiviruses accelerate experiments investigating exogenous gene overexpression phenotypes and downstream molecular impacts.
Product Type Lentiviral particle
Gene CSF1
Species Human
Titer Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc.
Size Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Summary Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.
Gene Name CSF1 colony stimulating factor 1 (macrophage) [ Homo sapiens ]
Gene Symbol CSF1
Synonyms MCSF; CSF-1
Gene Description colony stimulating factor 1 (macrophage)
Gene ID 1435
Uni Prot ID P09603
m RNA Refseq NM_172210.2
Protein Refseq NP_757349.1
Chromosome Location 1p13.3
Function cytokine activity; growth factor activity; growth factor activity; macrophage colony-stimulating factor receptor binding; macrophage colony-stimulating factor receptor binding; protein homodimerization activity;
Pathway Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytokines and Inflammatory Response, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Integrins in angiogenesis, organism-specific biosystem; Osteoclast differentiation, organism-specific biosystem;
MIM 120420
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