Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RR0031
Host Cell : CHO Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RR0031 |
| Introduction | The cyclic AMP response element (CRE)-binding protein (CREB) is probably one of the best understood phosphorylation dependent transcription factors. Increases in the concentration of either calcium or cAMP can induce activation of CREB, which leads to the transcription of target genes with cAMP responsive elements in their promoters. They regulate a wide range of biological functions, such as growth factor-dependent cell proliferation and survival, glucose homeostasis, spermatogenesis, circadian rhythms and memory. |
| Target Gene | CREB |
| Gene Species | Homo sapiens (Human) |
| Abbr | CHO-HuCREB Prom/Luciferase/Hyg |
| Host Cell | CHO |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Applications |
1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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