Human CDK2 Knockdown Cell Line-HeLa

Name: Human CDK2 Knockdown Cell Line-HeLa
SKU: CSC-RK0262

Cat.No. : CSC-RK0262

Host Cell: HeLa Validation: Real-Time RCR

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Cell Line Information

Safety and Packaging

Cat. No.	CSC-RK0262
Gene	CDK2
Alias	CDK2, p33(CDK2)
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Morphology	Epithelial
Stability	Validated for at least 10 passages
Application	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size Form	>1 × 10^6 cells / vial
Shipping	Dry ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

Publications

Q & A

Customer Reviews

The protein encoded by this gene is a member of the cyclin-dependent kinase family of Ser/Thr protein kinases. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, also known as Cdk1 in humans. It is a catalytic subunit of the cyclin-dependent kinase complex, whose activity is restricted to the G1-S phase of the cell cycle, and is essential for the G1/S transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin E or A. Cyclin E binds G1 phase Cdk2, which is required for the transition from G1 to S phase while binding with Cyclin A is required to progress through the S phase. Its activity is also regulated by phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. The role of this protein in G1-S transition has been recently questioned as cells lacking Cdk2 are reported to have no problem during this transition.

Customer Q&As

What are the three important functional domains of Cdks?

A: Cdks has three important functional domains. The first functional domain is the binding site of ATP and the active part of the enzyme; the second functional domain is the binding site of the regulatory subunit (Cyclin); the third functional domain is the binding site of P13suc1

How does Cdk drive cells to complete the cell cycle?

A: Various Cdk are activated at specific times during the cell cycle and drive cells to complete the cell cycle by phosphorylating substrates.

How does activated CDK1 keep the cell cycle running?

A: Activated CDK1 phosphorylates target proteins to produce corresponding physiological effects, such as phosphorylation of nucleocapsid proteins leading to nucleocapsid disassembly and loss of nuclear membrane, phosphorylation of H1 leading to condensation of chromosomes, etc. The end result of these effects keeps the cell cycle running.

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