Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-0997 Host Cell : HEK293
| Cat. No. | CLOE-0997 |
| Introduction | This cell lysate over-expresses extracellular domain of human CD74 isoform b (NP_004346.1) (Gln 73-Met 232) fused with a polyhistidine tag at the N-terminus. Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | CD74 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | CD74 CD74 molecule, major histocompatibility complex, class II invariant chain [ Homo sapiens ] |
| Gene Symbol | CD74 |
| Synonyms | CD74; CD74 molecule, major histocompatibility complex, class II invariant chain; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen associated) , DHLAG; HLA class II histocompatibility antigen gamma chain; gamma chain of class II antigens; HLA DR gamma; Ia associated invariant chain; MHC HLA DR gamma chain; p33; HLA-DR-gamma; MHC HLA-DR gamma chain; Ia-associated invariant chain; HLA-DR antigens-associated invariant chain; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); II; DHLAG; HLADG; Ia-GAMMA; FLJ98970; |
| Gene ID | 972 |
| Uni Prot ID | P04233 |
| m RNA Refseq | BC018726 |
| Chromosome Location | 5q31-q33 |
| Pathway | Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; Tuberculosis, organism-specific biosystem; Tuberculosis, conserved biosystem; |
| MIM | 142790 |
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