Human BUB1 Knockout Cell Line-Hela

Name: Human BUB1 Knockout Cell Line-Hela
SKU: CSC-RT2346
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2346

Target Gene : BUB1 Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2346
Description	This cell is a stable cell line with a homozygous knockout of human BUB1 using CRISPR/Cas9.
Target Gene	BUB1
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing
Storage	Liquid nirtogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:4-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) [ Homo sapiens ]
Gene Symbol	BUB1
Synonyms	BUB1; budding uninhibited; BUB1L, budding uninhibited by benzimidazoles 1 (yeast homolog); mitotic checkpoint serine/threonine-protein kinase BUB1; BUB1A; hBUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase; BUB1 budding uninhibited by benzimidazoles 1 homolog; BUB1L;
GeneID	699
Uni ProtID	O43683
mRNA Refseq	BC028201
Chromosome Location	2p11-q21
Function	ATP binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity;
Pathway	Aurora B signaling, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; DNA Replication, organism-specific biosystem;
MIM	602452

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Q & A

Customer Reviews

Customer Q&As

What is the recommended growth medium? Does it require antibiotic selection?

A: DMEM supplemented with 10% fetal bovine serum. <br> It is not required to add the selection antibiotics when culturing the KO cells.

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Worked very well

Human BUB1 KO cells help us study the function of genes. It's cool!

United States

2021-05-12

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Human BUB1 Knockout Cell Line-Hela

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Q & A

Customer Reviews

What is the recommended growth medium? Does it require antibiotic selection?

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Worked very well

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