Human ATG7 Knockout Cell Line-DLD-1

Name: Human ATG7 Knockout Cell Line-DLD-1
SKU: CSC-RT0024
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0024

Target Gene : ATG7 Host Cell : DLD-1

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT0024
Description	DLD-1-ATG7 (+/-) is a cell line with a heterozygous knockout of human ATG7
Target Gene	ATG7
Host Cell	DLD-1
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ATG7 ATG7 autophagy related 7 homolog (S. cerevisiae) [ Homo sapiens ]
Gene Symbol	ATG7
Synonyms	ATG7; ATG7 autophagy related 7 homolog (S. cerevisiae); APG7 autophagy 7 like (S. cerevisiae) , APG7L; ubiquitin-like modifier-activating enzyme ATG7; DKFZp434N0735; GSA7; hAGP7; autophagy-related protein 7; ATG12-activating enzyme E1 ATG7; ubiquitin activating enzyme E1-like protein; ubiquitin-activating enzyme E1-like protein; APG7L; APG7-LIKE;
Gene ID	10533
Uni Prot ID	O95352
m RNA Refseq	BC000091
Chromosome Location	3p25.3-p25.2
Function	APG12 activating enzyme activity; catalytic activity; nucleotide binding; protein binding; protein homodimerization activity; ubiquitin activating enzyme activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Regulation of autophagy, organism-specific biosystem;
MIM	608760

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Visualisation of results

My own experience with this reagent is that it works very well and is very intuitive, as can be seen from the experimental results.

United States

2023-03-14

Good result

This brand of reagent is more recognized by me, the famous brand can still have the corresponding effect, I highly recommend it to everyone to use, the effect can only be known if you have used it yourself.

United States

2023-01-22

Obvious result

I must exaggerate to say that this reagent saved my dying experiment, I had been unable to get the trend correct before and it made me doubt my experimental design, it was only after changing the reagent that I found it to be correct.

United States

2023-03-18

Convinced

This knockout reagent is available from many manufacturers, so why did I choose this brand, as I had used other reagents from this brand before and I felt it was more secure, and it turned out to be good.

United States

2023-03-19

Highly efficient

The transfection results were good and the results of the follow-up experiments were stable. I was very shocked to see a positive result on the first use, and I hope this result lasts longer.

United States

2023-01-15

Stable

I myself find the transfection reagents very stable and easy to use, other brothers and sisters in our lab have also used this brand of reagents and they have received unanimous praise.

United States

2023-03-04

Useful

My transfection results were good, and I did a western blot experiment to verify them, as well as the flow results, which were all usable results

United States

2023-03-21

Good results

I think the results of this experiment are still relatively good. After two weeks of use, the transfection efficiency is good and the conditions are now clearer for further experimentation.

United States

2023-03-26

Cheap and useful

My first time using this reagent did not work, it turned out that my cells were too sparse and I had better results the second time with experience.

United States

2023-01-25

Save energy

I think this would be considered the ideal transfection reagent among the cheaper reagents, cheap and with results, the best of both worlds.

United States

2023-02-12

Accurate

In terms of its transfection efficiency, it is more efficient, easier to produce results and saves time. Secondly, in terms of its price, it is really cheap.

United States

2023-02-12

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Human ATG7 Knockout Cell Line-DLD-1

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Q & A

Customer Reviews

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Visualisation of results

Good result

Obvious result

Convinced

Highly efficient

Stable

Useful

Good results

Cheap and useful

Save energy

Accurate

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