Cat.No. : CSC-RT0024
Host Cell: DLD-1 Target Gene: ATG7
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0024 |
| Description | DLD-1-ATG7 (+/-) is a cell line with a heterozygous knockout of human ATG7 |
| Target Gene | ATG7 |
| Host Cell | DLD-1 |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
My own experience with this reagent is that it works very well and is very intuitive, as can be seen from the experimental results.
This brand of reagent is more recognized by me, the famous brand can still have the corresponding effect, I highly recommend it to everyone to use, the effect can only be known if you have used it yourself.
I must exaggerate to say that this reagent saved my dying experiment, I had been unable to get the trend correct before and it made me doubt my experimental design, it was only after changing the reagent that I found it to be correct.
This knockout reagent is available from many manufacturers, so why did I choose this brand, as I had used other reagents from this brand before and I felt it was more secure, and it turned out to be good.
The transfection results were good and the results of the follow-up experiments were stable. I was very shocked to see a positive result on the first use, and I hope this result lasts longer.
I myself find the transfection reagents very stable and easy to use, other brothers and sisters in our lab have also used this brand of reagents and they have received unanimous praise.
My transfection results were good, and I did a western blot experiment to verify them, as well as the flow results, which were all usable results
I think the results of this experiment are still relatively good. After two weeks of use, the transfection efficiency is good and the conditions are now clearer for further experimentation.
My first time using this reagent did not work, it turned out that my cells were too sparse and I had better results the second time with experience.
I think this would be considered the ideal transfection reagent among the cheaper reagents, cheap and with results, the best of both worlds.
In terms of its transfection efficiency, it is more efficient, easier to produce results and saves time. Secondly, in terms of its price, it is really cheap.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
