Panoply™ Human ATG7 Knockdown Stable Cell Line

Name: Panoply™ Human ATG7 Knockdown Stable Cell Line
SKU: CSC-DC001106
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-DC001106

Host Cell : HEK293 (Hela and other cell types are also available) Validation : Real-Time RCR

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Cell Line Information

Safety and Packaging

Gene Information

Cat. No.	CSC-DC001106
Description	Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene	ATG7
Host Cell	HEK293 (Hela and other cell types are also available)
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid Nitrogen
Shipping	Dry Ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ATG7 autophagy related 7 [ Homo sapiens ]
Gene Symbol	ATG7
Synonyms	GSA7; APG7L; APG7-LIKE
Gene ID	10533
Uni Prot ID	O95352
m RNA Refseq	NM_001136031.2
Protein Refseq	NP_001129503.2
Chromosome Location	3p25.3
Function	APG12 activating enzyme activity; nucleotide binding; protein binding; protein homodimerization activity; ubiquitin activating enzyme activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Regulation of autophagy, organism-specific biosystem;
MIM	608760

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Customer Reviews

Customer Q&As

What is the role of ATG7 in autophagy?

A: ATG7 encodes a ubiquitin E1-like ligase that activates ATG12 prior to its binding to ATG5, promoting the expansion of prophagosomes.

What is the relationship between ATG7 and LC3-I and LC3-II?

A: ATG7 promotes the lipidation of the protein LC3-I with phosphatidylethanolamine to produce LC3-II, which is found on the inner and outer autophagosome membranes and recruits cytoplasmic cargo into autophagosomes either directly or via selective bridging proteins.

What is the relationship between mild myopathy and autophagy in ATG7-deficient human skeletal muscle?

A: ATG7 mutations cause congenital neurodevelopmental disorders due to impaired autophagy, reinforcing the association of ATG gene mutations in the autophagic system with congenital neurodevelopmental disorders

What are the characteristics of patients with mutations in the ATG7 gene?

A: Autosomal recessive, all patients present with a neurodevelopmental syndrome characterized by cerebellar hypoplasia, posterior corpus callosum thinning, ataxia, developmental delay, musculoskeletal abnormalities and facial dysmorphism

What is the biological function of ATG7?

A: An important example is ATG7, an essential autophagy effector enzyme that in concert with other ATG proteins, also regulates immunity, cell death and protein secretion, and independently regulates the cell cycle and apoptosis.

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