Cat.No. : CSC-DC001106
Host Cell: HEK293 (Hela and other cell types are also available) Validation: Real-Time RCR
| Cat. No. | CSC-DC001106 |
| Description | Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free. |
| Gene | ATG7 |
| Host Cell | HEK293 (Hela and other cell types are also available) |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | (1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Size Form | >1 × 10^6 cells / vial |
| Shipping | Dry Ice |
| Storage | Liquid Nitrogen |
| Gene Name | ATG7 autophagy related 7 [ Homo sapiens ] |
| Gene Symbol | ATG7 |
| Synonyms | GSA7; APG7L; APG7-LIKE |
| Gene ID | 10533 |
| UniProt ID | O95352 |
| mRNA Refseq | NM_001136031.2 |
| Protein Refseq | NP_001129503.2 |
| Chromosome Location | 3p25.3 |
| Function | APG12 activating enzyme activity; nucleotide binding; protein binding; protein homodimerization activity; ubiquitin activating enzyme activity; |
| Pathway | Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Regulation of autophagy, organism-specific biosystem; |
| MIM | 608760 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: ATG7 encodes a ubiquitin E1-like ligase that activates ATG12 prior to its binding to ATG5, promoting the expansion of prophagosomes.
A: ATG7 promotes the lipidation of the protein LC3-I with phosphatidylethanolamine to produce LC3-II, which is found on the inner and outer autophagosome membranes and recruits cytoplasmic cargo into autophagosomes either directly or via selective bridging proteins.
A: ATG7 mutations cause congenital neurodevelopmental disorders due to impaired autophagy, reinforcing the association of ATG gene mutations in the autophagic system with congenital neurodevelopmental disorders
A: Autosomal recessive, all patients present with a neurodevelopmental syndrome characterized by cerebellar hypoplasia, posterior corpus callosum thinning, ataxia, developmental delay, musculoskeletal abnormalities and facial dysmorphism
A: An important example is ATG7, an essential autophagy effector enzyme that in concert with other ATG proteins, also regulates immunity, cell death and protein secretion, and independently regulates the cell cycle and apoptosis.
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