Human ANPEP HEK293 Cell Lysate

Name: Human ANPEP HEK293 Cell Lysate
SKU: CLOE-1776
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1776 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1776
Introduction	This cell lysate over-expresses the human ANPEP (NP_001141.2) (Lys 69-Lys 967,603 Met/Ile) fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	ANPEP
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	ANPEP alanyl (membrane) aminopeptidase [ Homo sapiens ]
Gene Symbol	ANPEP
Synonyms	APN; CD13; LAP1; P150; PEPN; GP150
Gene Description	alanyl (membrane) aminopeptidase (aminopeptidase N, aminopeptidase M, microsomal aminopeptidase, CD13, p150)
Gene ID	290
Uni Prot ID	P15144
m RNA Refseq	NM_001150.2
Protein Refseq	NP_001141.2
Chromosome Location	15q25-q26
Function	aminopeptidase activity; metallopeptidase activity; peptide binding; receptor activity; zinc ion binding;
Pathway	C-MYB transcription factor network, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem; Glutathione metabolism, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Metabolism of Angiotensinogen to Angiotensins, organism-specific biosystem;
MIM	151530

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