Human ALPL HEK293 Cell Lysate

Name: Human ALPL HEK293 Cell Lysate
SKU: CLOE-1406
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1406 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1406
Introduction	This cell lysate over-expresses C-terminal polyhistidine tagged human ALPL (NP_000469.3) (Met 1-Ser 502). Purification of target protein (s) from this cell lysate has been verified.
Target Gene	ALPL
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	ALPL alkaline phosphatase, liver/bone/kidney [ Homo sapiens ]
Gene Symbol	ALPL
Synonyms	ALPL; alkaline phosphatase, liver/bone/kidney; HOPS; alkaline phosphatase, tissue-nonspecific isozyme; TNSALP; glycerophosphatase; tissue-nonspecific ALP; alkaline phosphomonoesterase; liver/bone/kidney-type alkaline phosphatase; alkaline phosphatase liver/bone/kidney isozyme; TNAP; APTNAP; AP-TNAP; FLJ40094; FLJ93059; MGC161443; MGC167935;
Gene ID	249
Uni Prot ID	P05186
m RNA Refseq	BC066116
Chromosome Location	1p36.12
Function	alkaline phosphatase activity; hydrolase activity; metal ion binding;
Pathway	Endochondral Ossification, organism-specific biosystem; Folate biosynthesis, organism-specific biosystem; Folate biosynthesis, conserved biosystem; Metabolic pathways, organism-specific biosystem; TNF-alpha/NF-kB Signaling Pathway, organism-specific biosystem;
MIM	171760

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