AhR Knockout Cell Line-H4IIE

Name: AhR Knockout Cell Line-H4IIE
SKU: CSC-RT0090
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0090

Target Gene : Ahr Host Cell : H4IIE

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT0090
Description	H4IIE-AhR cell line is a stable cell line with a homozygous knockout of AhR
Target Gene	Ahr
Host Cell	H4IIE
Host Cell Species	Rattus norvegicus (Rat)
Size	>1x10⁶ cells/vial
Validation	Sequencing

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Ahr aryl hydrocarbon receptor [ Rattus norvegicus ]
Gene Symbol	Ahr
Gene Description	aryl hydrocarbon receptor
GeneID	25690
Uni ProtID	P41738
mRNA Refseq	NM_013149.2
Protein Refseq	NP_037281.2
Chromosome Location	6q16
Function	DNA binding; DNA binding; Hsp90 protein binding; Hsp90 protein binding; RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; aryl hydrocarbon receptor activity; aryl hydrocarbon receptor activity; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; protein binding; protein dimerization activity; protein heterodimerization activity; sequence-specific DNA binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor activity; transcription factor binding; transcription factor binding; transcription regulatory region DNA binding; transcription regulatory region DNA binding;
Pathway	Adipogenesis, organism-specific biosystem;

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Exceeded my expectations

AhR Knockout Cell Line-H4IIE has exceeded my expectations in terms of knockdown efficiency and ease of use. It has become an invaluable asset in my research endeavors.

United States

2023-04-01

Exceptional performance

I highly recommend AhR Knockout Cell Line-H4IIE to fellow researchers. Its exceptional performance and consistent results have significantly advanced my understanding of gene function.

United States

2020-08-23

Remarkable precision

Using AhR Knockout Cell Line-H4IIE has been a competent assistants for my experiments. It has empowered me to unravel the intricate mechanisms underlying gene regulation with remarkable precision.

United States

2021-05-12

High efficiency and reproducibility

AhR Knockout Cell Line-H4IIE has simplified the complex process of gene editing. Its high efficiency and reproducibility have empowered me to achieve reliable results, enabling groundbreaking discoveries.

United States

2021-08-15

Unparalleled

I am absolutely thrilled with the results I've achieved using AhR Knockout Cell Line-H4IIE. It has provided me with unparalleled control over gene expression, leading to groundbreaking discoveries.

Germany

2021-10-09

Precise and efficient

AhR Knockout Cell Line-H4IIE has transformed my research by enabling precise and efficient gene editing. Its exceptional performance has saved me time and resources while yielding remarkable outcomes.

United States

2022-01-27

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AhR Knockout Cell Line-H4IIE

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Q & A

Customer Reviews

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Exceeded my expectations

Exceptional performance

Remarkable precision

High efficiency and reproducibility

Unparalleled

Precise and efficient

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