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Powerful Tn5 Transposase for DNA insertion and random library construction.
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Cat.No. : VNV-026
Storage: -80°C Shipping: Dry ice
| Cat. No. | VNV-026 |
| Description | Wild-type parainfluenza virus type 2 particles which are inactivated by β-Propiolactone treatment. This product is intended for research use only. |
| Shipping | Dry ice |
| Storage | -80°C |
| Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. | |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
A: The products are primarily used for research purposes, such as studying the virus's molecular characteristics, mechanisms of infection, or potential antiviral therapies. They can also be used in diagnostic assays to detect the presence of Parainfluenza Virus 2 in biological samples.
A: Creative Biogene employs several quality control measures to ensure the purity and stability of our Wild-Type Parainfluenza Virus 2 products. These measures include genetic sequencing,physicochemical characterization,sterility testing,endotoxin testing and stability studies.
A: PIVs are characterised by producing enveloped virions and containing single-stranded negative sense RNA. Non-infectious virions have also been reported to contain RNA with positive polarity. The structural gene sequence of PIVs is as follows: 3′-NP-P-M-F-HN-L-5′.
A: Symptoms of a PIV-2 infection include fever, cough, runny nose, sore throat, and breathing difficulties. In severe cases, it can lead to bronchiolitis or pneumonia, especially in infants and people with weakened immune systems.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
I am extremely satisfied with Wild-Type Parainfluenza Virus 2's viral titer. The titer was impressively high, ensuring a strong and effective product.
United States
03/14/2022
The virus was isolated and characterized with great precision, ensuring its identity and purity.
United States
10/07/2022
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