Cat.No. : CSC-RR0480 Host Cell: Ba/F3
| Cat. No. | CSC-RR0480 |
| Description | STAT6-Luc Reporter Cell Line-Ba/F3 stably expresses luciferase reporter gene under the control of the STAT6 responsive promoter. This cell line is an ideal cellular model for monitoring STAT6 signaling pathway activities. |
| Gene | STAT6-Luc |
| Host Cell | Ba/F3 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The STAT6-Luc Reporter Cell Line - Ba/F3 is specifically designed for the study of cytokine signaling, particularly for signals that activate the STAT6 pathway, such as IL-4 and IL-13 signaling in immune responses. It's extensively used to investigate the activation and regulation of STAT6-dependent gene expression, identify and characterize cytokines and other molecules that modulate STAT6 signaling, and screen for potential therapeutic agents that target STAT6 pathways in diseases like asthma, allergic responses, and certain forms of cancer.
A: Researchers can measure STAT6 activation in the STAT6-Luc Reporter Cell Line - Ba/F3 by detecting luciferase activity. Upon stimulation of the STAT6 pathway, the luciferase reporter gene is activated, leading to luciferase enzyme production. The enzyme's activity can be quantitatively measured using a luminometer after adding a luciferase substrate, providing a direct readout of STAT6 pathway activation. This method allows for the sensitive and quantitative analysis of STAT6 activity in response to various stimuli or inhibitors.
A: To ensure reliability and reproducibility, experiments with the STAT6-Luc Reporter Cell Line - Ba/F3 should include proper controls, such as cells treated with known activators or inhibitors of STAT6 for comparison. It's also crucial to maintain consistent cell culture conditions, use freshly prepared reagents, and standardize luciferase assay procedures. Performing assays in triplicates or more can help in averaging out variability, and validating results with complementary techniques like Western blotting for STAT6 phosphorylation can further confirm the findings.
A: Challenges in working with the STAT6-Luc Reporter Cell Line - Ba/F3 include maintaining stable luciferase expression and preventing cell line contamination. To address these challenges, the cell line should be regularly tested for mycoplasma contamination and luciferase gene integrity. Additionally, using selective antibiotics can help maintain the selection pressure for the reporter construct. Implementing strict cell culture practices will minimize the risk of contamination and genetic drift, ensuring the cell line's long-term stability and reliability.
A: The STAT6-Luc Reporter Cell Line - Ba/F3 can be utilized to evaluate the efficacy of novel anti-inflammatory compounds by measuring their impact on STAT6 pathway activation. Compounds of interest can be screened for their ability to inhibit cytokine-induced STAT6 activation, as evidenced by a reduction in luciferase activity. This approach enables the identification of compounds that could potentially be developed into therapies for diseases characterized by aberrant STAT6 signaling, such as certain inflammatory and autoimmune conditions, by providing a high-throughput and sensitive platform for early-stage drug discovery.
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The Ba/F3 cell line stably expressing a STAT6-driven luciferase reporter allows for highly sensitive detection of STAT6 activation, which is crucial for studying the signaling pathways involved in immune responses and cancer biology.
This cell line enables rapid assessment of STAT6 activity upon stimulation, facilitating the quick evaluation of potential agonists or antagonists targeting the JAK-STAT signaling pathway.
The STAT6-Luc Reporter Cell Line - Ba/F3 is ideal for developing standardized cell-based assays, which are essential for preclinical drug discovery and validation of compounds affecting STAT6 signaling.
Working with a clonal cell line like Ba/F3 ensures a homogeneous population for experiments, reducing variability and providing more consistent and reliable data in STAT6 signaling research.
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