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pUMVC

For research use only. Not intended for any clinical use.
Cat.No.
OVT2740
Host Cell
Mammalian cells, Escherichia coli
Product Type
Vector
Resistance
Kanamycin
Vector Size
9287 bp

Background

Applications

Publications

Q & A

Customer Reviews

pUMVC is a mammalian vector and a retrovirus packaging vector, part of a dual plasmid packaging system. It falls under the category of low-copy vectors, meaning there are fewer replicas of the DNA being cloned in each bacterium. The technique used for cloning involves restriction enzymes and multiple cloning sites, which allows a variety of different sequences to be inserted into the vector in one step. The promoter in pUMVC is CMV or Cytomegalovirus, which is a commonly used viral promoter for high-level, constitutive expression in a broad range of mammalian cells. The entire vector measures 9287 base pairs in size. The cloning strains suitable for pUMVC include DH5α. The host cells (lines) being used are packaging cell lines like 293T.
pUMVC vectors are utilized in the field of genetic research and molecular biology. They are tools for gene cloning, gene expression, gene silencing, and the creation of knockout organisms. Here are some applications of pUMVC vectors: Gene Cloning: The pUMVC vector is used for gene cloning in genetic research. In this procedure, the desired gene is inserted into the pUMVC vector to create recombinant DNA. The vector then carries the inserted gene into the host organism. Gene Expression Studies: Researchers often use pUMVC vectors to study gene expression in different organisms. This involves introducing a gene into the vector and monitoring its expression levels in the organism. Defining Gene Function: By disrupting the normal function of a gene, pUMVC vectors can be used to determine its role. This is achieved by creating a mutation in the gene, which the vector then carries into the host organism. Creation of Knockout Organisms: pUMVC vectors can also be used to create knockout organisms. The vectors are used to disrupt specific genes in a host organism. This results in the "knockout" of the gene, which can be helpful in studying diseases associated with genetic mutations.
Customer Q&As
What is the length of pUMVC?

A: The length of pUMVC is 9287 bp.

What is the recommended envelope plasmid to use in conjunction with pUMVC?

A: The recommended envelope plasmid to use in conjunction with pUMVC is pCMV-VSV-G.

What is the type of pUMVC?

A: The type of pUMVC is viral expression & packaging vectors.

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Customer Reviews
High Efficiency

pUMVC vector has been engineered for high level of viral production which allows researchers to achieve the high level of transgene expression in their experiments.

United Kingdom

09/03/2020

Valuable tool

pUMVC vector is especially useful when dealing with cells that are hard to transfect.

United Kingdom

07/26/2020

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