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PRV-ΔTK-hUbC-DsRed

For research use only. Not intended for any clinical use.

Cat. No. :   VNTP-4

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Virus Particles Information

Cat. No. VNTP-4
Description Replication-incompetent retrograde monosynaptic PRV Bartha tracer with the thymidine kinase (TK, UL23) gene deleted. Expresses DsRed under the human ubiquitin C (hUbC) promoter. Because TK is essential for viral genome replication in neurons, this tracer cannot replicate or cross synapses alone. When co-injected at the same site with an AAV helper virus (e.g., AAV-hUbC-TK or AAV-DIO-TK) that provides TK in trans, the virus replicates only in helper-transduced starter neurons and crosses a single synapse to label directly presynaptic neurons, enabling monosynaptic input mapping.
Product Type Pseudorabies virus
Reporter DsRed
Synaptic Transmission Mono-synaptic (single-step, with AAV-TK helper)
TKStatus TK-deleted (dTK, replication-incompetent alone)
Applications
  1. Retrograde monosynaptic tracing to identify direct first-order presynaptic inputs of a starter population (requires co-injection with AAV-TK helper)
  2. Cell-type-specific input mapping when combined with AAV-DIO-TK in Cre-driver lines
  3. Mapping direct afferents to a defined brain region
  4. Delineating monosynaptic connectomes in neuropsychiatric, autonomic, and sensory circuits.
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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