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pRSV-Rev

For research use only. Not intended for any clinical use.
Cat.No.
OVT2821
Host Cell
Mammalian cells, Escherichia coli
Product Type
Vector
Promoter
RSV U3
Resistance
Ampicillin
Vector Size
4174 bp

Background

Applications

Publications

Q & A

Customer Reviews

The pRSV-Rev vector is a highly efficacious viral expression and packaging vector. It comes with a high copy number, signifying enhanced yields during the production phase. Transcriptional units residing within the vector possess a length of roughly 4174 base pairs. As such, they can accommodate sizable portions of genetic material required for diverse gene therapy applications and research purposes. One of the unique features of the pRSV-Rev vector is its antibiotic resistance towards Ampicillin, a prevalent antibiotic used in research. The incorporation of the resistance marker allows the growth and propagation of only the cells that carry the vector- providing researchers with a greater degree of certainty in successful cloning or plasmid extraction. It is worth noting that the pRSV-Rev vector functions as the 3rd generation lentiviral packaging plasmid. This model offers pronounced enhancements over earlier iterations, offering simplified and more effective gene transfers. However, it is essential to mention that for successful packaging and subsequent expression, the presence of pMDLg/pRRE and a suitable envelope expressing plasmid is a requisite.
pRSV-Rev vector is primarily used in scientific research for gene transfer in various cellular systems. Its applications are majorly in molecular biology, genetics, biochemistry, and pharmaceutical research. Here are some of its specific applications: 1) Gene Therapy: pRSV-Rev vector is used in gene therapy for delivery of specific genes into host cells. It is used to manipulate the genetic information of cells which helps in the treatment of diseases. 2) Intracellular Studies: By introducing a selected gene into a cell, scientists can study the effects and roles of that particular gene within a cell. This can give insights into the functionalities of genes, including their contributions to certain diseases. 3) Protein Production: In biotechnology and pharmaceutical industry, the vector is used to produce large amounts of specific proteins by inserting the desired gene into host cells. 4) Vaccine Development: The pRSV-Rev vector can be utilized in the development of new vaccines by introducing antigen genes into host cells, causing them to produce the antigen and elicit an immune response. 5) Study of Viruses: As the pRSV-Rev vector contains elements for replication and packaging of retroviruses, it can be used to study the biology and life cycle of retroviruses.
Customer Q&As
What is the size of the pRSV-Rev vector?

A: The size of the pRSV-Rev plasmid is 4180 vector.

Which strain was cloned to create the pRSV-Rev plasmid?

A: The pRSV-Rev plasmid was cloned in the Stbl3 strain.

What are the sequencing primers used for the 5' end of the pRSV-Rev vector?

A: The sequencing primer for the 5' end of the pRSV-Rev vector is M13R:CAGGAAACAGCTATGACC.

What is the Rev protein?

A: Rev protein is an important HIV regulatory protein that binds to the RRE (Rev response element) sequence in the viral RNA genome and is critical for HIV-1 replication.

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Customer Reviews
Stable Replication

pRSV-Rev vector is derived from a stable parent vector, facilitating consistent results in repeated experiments.

United Kingdom

04/04/2021

Ease of Use

pRSV-Rev vector is easy to include, manipulate, and remove gene sequences, which allows us to conveniently work with the vector in various types of experiments.

French

08/11/2021

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