Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : OVT2790
| Cat. No. | OVT2790 |
| Product Type | Vector |
| Host Cell | Mammalian cells, Escherichia coli |
| Resistance | Ampicillin |
| Vector Size | 8931 bp |
The pRRLSIN.CPPT.PGK-GFP.WPRE vector is a lentiviral cloning vector, characterized by its size of 8931 base pairs. It is part of the viral series and is predominantly used in mammalian cells for expression.
The vector employs several promoters for its action. The human phosphoglycerate kinase (hPGK) promoter is used for internal gene expression. Both the T3 and T7 promoters allow control over transcription in vitro. Moreover, the vector possesses a broad host range due to the presence of multiple origin of replication sites. It can replicate in bacterial cells using the pUC ori and f1 ori and in eukaryotic cells employing the SV40 ori. The termination of transcription is programmed via the SV40 poly(A) signal. This signal ensures that the generated RNA molecules are appropriately processed and exported from the nucleus to the cytoplasm, primed and ready for protein synthesis.
Going further, the pRRLSIN.CPPT.PGK-GFP.WPRE vector possesses a green fluorescent protein (GFP) gene, which gets expressed in host cells without the need for inducement. Its expression is transient, thus making it useful for monitoring the plasmid delivery into the cells.
The pRRLSIN.cPPT.PGK-GFP.WPRE vector is a highly useful tool in medical research and genetic engineering.
Genetic Research: The vector is widely used in laboratories for creating transgenic organisms, whereby a foreign gene (GFP in the case of this vector) is inserted into the genome of the organism in question. This allows scientists to study the function and behavior of the particular gene in a controlled and observable environment.
Cell Labelling: The pRRLSIN.cPPT.PGK-GFP.WPRE vector carries the GFP (Green Fluorescent Protein) gene, which encodes a protein that fluoresces green whenever it is exposed to blue light. This has made the vector incredibly useful for biologists to use as a molecular tag. When the GFP gene is attached to another gene of interest, scientists can visually track the location, movement, and interaction of the proteins produced by that gene within the cell.
Drug Development: As drug development increasingly targets genetic and cellular mechanisms, the vector is also of high value in pharmaceutical research. Through its use, researchers could assess the impacts of potential drugs on the gene expression and cellular processes in a controlled manner.
Gene Knockdown Studies: In addition to gene overexpression studies, this vector can also be used for gene knockdown studies by incorporating RNA interference (RNAi) technology. Through this, researchers can better understand the function of a particular gene by observing the biological effects when the gene is silenced.
A: The pRRLSIN.cPPT.PGK-GFP.WPRE codes for GFP (green fluorescent protein) under the control of the PGK promoter, flanked by psi sequences and HIV-1 long terminal repeats.
A: The flanking psi sequences serve to enhance the packaging efficiency of the plasmid during viral particle production.
A: The WPRE sequence is a posttranscriptional regulatory element derived from the woodchuck hepatitis virus, which helps increase transgene expression levels in the target cells.
A: The pRRL was derived from a plasmid made by the Naldini lab.
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The pRRLSIN.cPPT.PGK-GFP.WPRE vector is a highly reliable tool for efficient transgene delivery and stable expression in dividing and non-dividing cells.
The pRRLSIN.cPPT.PGK-GFP.WPRE vector utilizes the powerful features of the cPPT element, ensuring nuclear transport and subsequent integration of the viral genome.
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