Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-SC004625-1
Host Cell : HEK293 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-SC004625-1 |
| Description | The overexpression cassette containing human DUX4 gene driven by a tetracycline inducible promoter (Tet-On system) was stably integrated into the HEK293 genome. Human DUX4 expression is rapidly induced in the presence of tetracycline (Tc) or tetracycline-derivatives like doxycycline (dox). |
| Target Gene | DUX4 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 frozen cells/vial |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | DUX4 double homeobox 4 [ Homo sapiens ] |
| Gene Symbol | DUX4 |
| Synonyms | DUX10 |
| GeneID | 100288687 |
| Uni ProtID | F5GZ66 |
| mRNA Refseq | NM_033178.2 |
| Protein Refseq | NP_149418.3 |
| Chromosome Location | 4q35 |
| Function | sequence-specific DNA binding transcription factor activity; transcription regulatory region sequence-specific DNA binding; |
| MIM | 606009 |
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No leaky expression in the off state and strong induction with doxycycline. Perfect for our DUX4 toxicity studies—clean results every time. Highly recommended.
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