Tet/on-Human DUX4 Stable Cell Line-HEK293

Name: Tet/on-Human DUX4 Stable Cell Line-HEK293
SKU: CSC-SC004625-1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC004625-1

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC004625-1
Description	The overexpression cassette containing human DUX4 gene driven by a tetracycline inducible promoter (Tet-On system) was stably integrated into the HEK293 genome. Human DUX4 expression is rapidly induced in the presence of tetracycline (Tc) or tetracycline-derivatives like doxycycline (dox).
Target Gene	DUX4
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ frozen cells/vial
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	DUX4 double homeobox 4 [ Homo sapiens ]
Gene Symbol	DUX4
Synonyms	DUX10
Gene ID	100288687
Uni Prot ID	F5GZ66
m RNA Refseq	NM_033178.2
Protein Refseq	NP_149418.3
Chromosome Location	4q35
Function	sequence-specific DNA binding transcription factor activity; transcription regulatory region sequence-specific DNA binding;
MIM	606009

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