tdTomato MSCV Retrovirus

Name: tdTomato MSCV Retrovirus
SKU: RV00008Z

Cat.No. : RV00008Z

Titer: ≥1*10^7 TU/mL / ≥1*10^8 TU/mL Size: 100 ul/500 ul/1 mL

Storage: -80℃ Shipping: Frozen on dry ice

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Lentivirus Particle Information

Quality Control

Cat. No.	RV00008Z
Description	Premade MSCV-based, VSV-G pseudotyped retroviral particles that contain tdTomato reporter gene.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Creative Biogene ensures high-quality retrovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between retrovirus particle lots.
Mycoplasma	Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that retrovirus products are free of mycoplasma contamination.
Purity	Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared retrovirus vectors to ensure they meet quality standards.
Sterility	The retrovirus samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the retrovirus products are free of microbial contamination.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of retrovirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation	All Creative Biogene retrovirus vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the retrovirus vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected retrovirus insert.

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