tdTomato/Luc Reporter Cell Line - NUGC4

Name: tdTomato/Luc Reporter Cell Line - NUGC4
SKU: CSC-RR0637-2
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0637-2

Host Cell : NUGC4 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RR0637-2
Description	This cell line is engineered to stably co-express tdTomato and luciferase reporter genes in human gastric signet ring cell adenocarcinoma NUGC4.
Product Type	Stable cell line constitutively co-expressing tdTomato and luciferase reporter genes
Target Gene	tdTomato/Luc
Host Cell	NUGC4
Host Cell Species	Homo sapiens (Human)
Reporter	tdTomato and luciferase reporter genes
Applications	1) in vitro cell tracking by bioluminescent and/or fluorescent signal 2) monitor in vivo tumor growth using the bioluminescence and/or fluorescent signal 3) anticancer drug development
Resistance	Blasticidin
Size	One vial of frozen cells, typically >1x10^6cells/vial
Stability	This cell line is stable at least 10 passages.
Quality Control	1) Luciferase activity assay and detection of tdTomato fluorescence under microscopy 2) Mycoplasma detection
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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