T7p07 Stable Cell Line - BHK-21

Name: T7p07 Stable Cell Line - BHK-21
SKU: CSC-RO01375

Cat.No. : CSC-RO01375 Host Cell: Baby Syrian Hamster kidney fibroblast cell line (BHK-21)

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Cell Line Information

Cell Culture Information

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Cat. No.	CSC-RO01375
Description	This cell line is engineered to stably express Escherichia phage T7 RNA polymerase (T7p07) in Baby Syrian Hamster kidney fibroblast cell line (BHK-21). mCherry reporter gene is also expressed in this cell line allowing fluorescent tracking of cells.
Reporter	mCherry
Gene	T7p07
Gene Species	Escherichia phage T7
Host Cell	Baby Syrian Hamster kidney fibroblast cell line (BHK-21)
Host Cell Species	Syrian hamster (Mesocricetus auratus) cell line
Stability	This cell line is stable at least 10 passages.
Product Type	Escherichia phage T7 gene overexpression stable cell line
Applications	1) investigation of gene function 2) screening and validation of antibodies
Quality Control	1) Real-time qPCR analysis of gene mRNA overexpression level 2) mCherry fluorescent detection under fluorescent microscopy 3) mycoplasma detection
Size Form	One vial of frozen cells, typically >1x10^6cells/vial
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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