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Src Easy KO Kit (Human)

For research use only. Not intended for any clinical use.
Cat.No.
CC-1391
Description
A complete kit for efficient gene knockout in mammalian cells, providing chemically synthesized sgRNAs and PCR/sequencing primers for targeted DNA cleavage and gene disruption. Cas9 protein and transfection reagents need to be supplied by the user. All essential reagents for knockout validation are included for rapid and accurate gene editing.
Applications
This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs targeting the selected gene. When combined with user-supplied Cas9 protein and transfection reagents, transfected sgRNAs guide CRISPR-mediated cleavage of early exons, inducing deletions or frameshift mutations for efficient and rapid knockout.
Features
  • Ready-to-use sgRNAs and primers—no need for design or validation.
  • Compatible with standard Cas9 proteins and transfection reagents.
  • Enables fast, accurate gene knockout with minimal optimization.
  • Cost-effective option for users with an existing genome-editing setup.
Species
Human
Storage
Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.
Classification
Gene-specific KO Kit
NCBI Gene ID
6714
Ensembl ID
ENSG00000197122
UniProt ID
P12931
GeneAbbr
SRC SRC1
Reactions
5–10 reactions per target gene
Kit Components
  • 2–3 chemically synthesized sgRNAs (200 pmol each)
  • 3 PCR/Sequencing primers (500 pmol each)
  • LM cell lysate (500 µL)

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