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Nluc Reporter Cell Line - NCI-H660

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RR01225

Host Cell :   NCI-H660 Size :   >1x106 frozen cells/vial

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Cat. No. CSC-RR01225
Description This cell line is engineered to stably exprress NanoLuc Luciferase(NLuc) reporter gene in NCI-H660 cells. It is a useful tool for bioluminescent tracking of NCI-H660 cells.
Product Type Bioluminescent Reporter Cell Lines
Target Gene Nluc
Host Cell NCI-H660
Host Cell Species Homo sapiens (Human)
Applications in vitro cell tracking and in vivo cell imaging
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent cell line
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
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Customer Reviews
Superior brightness and sensitivity

Creative Biogene’s Nluc version of the NCI-H660 line exceeded our expectations. The NanoLuc signal is significantly brighter than standard luciferase, which allowed us to detect even small changes in cell viability during our drug screening assays. The cells arrived healthy and established quickly in culture.

Germany

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