NFκB-Luc/EF1α-mCherry Reporter Cell Line - SGC-7901

SGC-7901 is a human gastric adenocarcinoma cell line originally isolated from a metastatic lymph node of a female patient with advanced stomach cancer. It grows as an adherent epithelial monolayer and is widely used as a representative gastric cancer model for studying tumor proliferation, drug response, and inflammation-linked signaling. The NFκB-Luc/EF1α-mCherry Reporter Cell Line - SGC-7901 is built on this background by stably integrating a firefly luciferase reporter driven by multiple NFκB response elements, together with an mCherry fluorescent marker and a puromycin resistance cassette under the constitutive EF1α promoter. The dual readouts (luciferase for quantitative signal, mCherry for visual confirmation of successful transduction and cell viability) make it easy to verify NFκB pathway activation in real time without the need for additional staining or lysis-based normalization controls.

This reporter line is most often deployed in inflammation, oncology, and drug discovery workflows that require sensitive monitoring of NFκB activity in a gastric cancer context. Common applications include screening small-molecule inhibitors or natural-product libraries that target upstream kinases (IKK, TAK1) or downstream regulators of the canonical NFκB cascade, evaluating the inflammatory effects of cytokines such as TNF-α, IL-1β, or LPS in a tumor-relevant background, and characterizing the crosstalk between NFκB and other oncogenic pathways active in gastric cancer. Because the line carries an mCherry internal control, it is also useful for normalization in high-content imaging, for tracking tumor cells in co-culture or invasion assays, and as a stable background for CRISPR or RNAi-based target validation studies where baseline NFκB readouts need to remain consistent across passages.