mScarlet/LUC Reporter Cell Line - T24

T24 is a human urinary bladder carcinoma cell line established from a high-grade transitional cell carcinoma and is one of the most extensively studied models in bladder cancer research. The cell line is characterized by aggressive proliferative capacity, invasive behavior, and activating mutations in oncogenic signaling pathways, making it highly suitable for investigating mechanisms of urothelial tumor development and progression. T24 cells have been widely utilized in studies of tumor biology, metastasis, drug resistance, and molecular therapeutic targets. Due to their robust tumorigenic potential and reproducible growth characteristics, T24 cells serve as a valuable platform for both basic and translational cancer research. The mScarlet/LUC Reporter Cell Line - T24 is generated through stable introduction of the red fluorescent protein mScarlet and firefly luciferase (LUC) reporter genes into parental T24 cells. This dual-reporter system enables sensitive fluorescence imaging and quantitative bioluminescence monitoring while maintaining the biological characteristics and tumorigenic properties of the original cell line.

The T24-mScarlet/LUC reporter cell line is widely employed in bladder cancer research for real-time evaluation of tumor growth, invasion, metastasis, and therapeutic response. Luciferase expression enables non-invasive, longitudinal monitoring of tumor burden and disease progression through bioluminescence imaging in xenograft models, allowing accurate assessment of treatment efficacy over time. Simultaneously, mScarlet expression provides bright and stable red fluorescence for high-resolution visualization of cellular morphology, proliferation, migration, invasion, and tissue localization. This model is particularly useful for screening anti-cancer compounds, evaluating targeted therapies and immunotherapeutic approaches, and investigating mechanisms of therapeutic resistance in urothelial carcinoma. In addition, the dual-reporter platform supports studies of epithelial–mesenchymal transition, tumor–microenvironment interactions, cancer stem cell-associated phenotypes, and signaling pathways involved in bladder cancer progression and metastasis. With stable reporter expression and robust imaging capabilities, the T24-mScarlet/LUC cell line provides a reliable and reproducible tool for preclinical drug development and translational research in bladder cancer.