Mouse Pvalb Stable Cell Line - HT-22

The Pvalb gene encodes parvalbumin, a highly conserved, low-molecular-weight calcium-binding protein belonging to the EF-hand superfamily. In the mammalian central nervous system, parvalbumin is predominantly expressed in a specific subset of fast-spiking GABAergic inhibitory interneurons. These interneurons are critical for coordinating and synchronizing cortical neural network activity, as well as for generating gamma oscillations. The primary physiological function of parvalbumin is to act as an intracellular "slow" calcium buffer. By binding to calcium ions that enter the cytoplasm during action potentials, parvalbumin accelerates the decay of calcium transients, thereby facilitating rapid, repetitive neuronal firing and protecting neurons from potential calcium overload and the ensuing excitotoxic damage. Given its pivotal role in regulating calcium homeostasis and neuronal excitability, the Pvalb gene is considered to be closely implicated in the pathogenesis of various complex neurological and psychiatric disorders. Extensive neurobiological research has confirmed that alterations in parvalbumin expression levels—or dysfunction of parvalbumin-positive interneurons—are intimately linked to the underlying mechanisms of diseases such as schizophrenia, autism spectrum disorder, epilepsy, and neurodegenerative conditions like Alzheimer''s disease.

The "Mouse Pvalb Stable Cell Line," established within an HT-22 cell background, represents a highly valuable and sophisticated in vitro research tool specifically designed to advance investigations into the mechanisms governing parvalbumin''s role in neuronal function and survival. The HT-22 cell line is an immortalized murine hippocampal neuronal cell line, originally derived from the HT-4 cell line. By stably integrating the mouse Pvalb gene into the HT-22 cell genome, this genetically engineered cell line ensures consistent, sustained, and uniform parvalbumin expression levels across successive cell passages, thereby effectively circumventing the issues of expression instability and low efficiency often associated with transient transfection techniques. The murine Pvalb stable cell line enables scientists to investigate how the enhanced buffering capacity conferred by parvalbumin influences calcium signaling pathways, mitochondrial function, and cellular resilience under various stress conditions. Furthermore, the murine Pvalb-expressing HT-22 stable cell line is widely utilized in the field of neuropharmacology for the high-throughput screening of novel neuroprotective compounds—particularly those designed to ameliorate intracellular calcium dysregulation and oxidative damage.