Mouse Prlr Stable Cell Line - HT-22

The Prlr gene encodes the prolactin receptor, a critical transmembrane protein belonging to the Class I cytokine receptor superfamily. Structurally, the receptor consists of an extracellular ligand-binding domain, a single transmembrane region, and an intracellular domain. Notably, the intracellular domain itself lacks intrinsic tyrosine kinase activity; consequently, it must rely on associated intracellular kinases to mediate signal transduction. This receptor mediates a diverse array of physiological functions of prolactin. Upon binding with prolactin, the prolactin receptor undergoes conformational changes and dimerizes, thereby triggering a complex cascade of intracellular signaling events. In mice, the Prlr gene undergoes alternative splicing to generate various receptor isoforms; these isoforms are expressed across a wide spectrum of tissue types, with a distribution ranging from classic reproductive organs and mammary glands to the liver, immune cells, and the central nervous system. In the mammalian brain, the functional significance of prolactin receptor signaling has garnered increasing attention, extending far beyond the traditional scope of reproductive physiology; for instance, it plays a pivotal and fundamental role in regulating maternal behavior, promoting adult neurogenesis in the olfactory bulb and hippocampus, attenuating physiological stress responses, and conferring significant neuroprotection against various pathological insults.

The "Mouse Prlr Stable Cell Line," established within the background of HT-22 cells, represents a highly specific and invaluable research model. It was designed specifically to advance investigations into the mechanisms of prolactin receptor signaling, particularly within the critical context of the nervous system. The parental cell line for this stable cell model—the HT-22 cell line—is an immortalized murine hippocampal neuronal cell line. This cell line is widely utilized in neurobiological research due to its ability to retain key intrinsic biological characteristics of neuronal cells, as well as its well-documented high sensitivity to glutamate-induced oxidative stress. By stably integrating and expressing the gene encoding the murine prolactin receptor within these hippocampal neuronal cells, this genetically engineered cell line establishes a robust, sustained, and highly responsive research platform. It effectively eliminates the drawbacks—such as high variability and low transfection efficiency—often associated with traditional transient transfection methods. Researchers can extensively utilize this stable cell line to pursue a wide range of cutting-edge applications, including high-throughput screening assays designed to systematically identify novel prolactin receptor agonists, antagonists, or subtle allosteric modulators.