Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RO01361
Host Cell : RAW264.7 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RO01361 |
| Description | This cell line is engineered to stably express Mus musculus (Murine, House mouse) prohibitin 2 (Phb2) in Mouse macrophage cell line (RAW 264.7). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells. |
| Product Type | Mouse gene overexpression stable cell line |
| Target Gene | Phb2 |
| Gene Species | Mus musculus (Murine, House mouse) |
| Host Cell | RAW264.7 |
| Host Cell Species | Mus musculus (Mouse) |
| Reporter | GFP |
| Applications |
1) investigation of gene function 2) screening and validation of antibodies |
| Size | One vial of frozen cells, typically >1x10^6cells/vial |
| Stability | This cell line is stable at least 10 passages. |
| Quality Control |
1) Real-time qPCR analysis of gene mRNA overexpression level 2) GFP fluorescent detection under fluorescent microscopy 3) mycoplasma detection |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Target Gene | PHB2 |
| Background | Enables amide binding activity; protein dimerization activity; and sphingolipid binding activity. Involved in several processes, including RIG-I signaling pathway; positive regulation of cell cycle phase transition; and regulation of DNA-templated transcription. Located in several cellular components, including cell surface; mitochondrial membrane; and nuclear matrix. Part of mitochondrial prohibitin complex. [provided by Alliance of Genome Resources, Feb 2025] |
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The Mouse Phb2 Stable Cell Line thawed cleanly, expanded quickly, and showed consistent PHB2 expression across passages. Shipment arrived cold and well-packed. Technical support was prompt and helpful with optimization tips.
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