Mouse Otof Stable Cell Line - HEK293T

Name: Mouse Otof Stable Cell Line - HEK293T
SKU: CSC-RO01392

Cat.No. : CSC-RO01392 Host Cell: Human embryonic kidney immortal cell line transformed with SV40 large T antigen (HEK293T)

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Gene Informationn

Cat. No.	CSC-RO01392
Description	This cell line is engineered to stably express Mus musculus (Murine, House mouse) otoferlin (Otof) in Human embryonic kidney immortal cell line transformed with SV40 large T antigen (HEK293T). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells.
Reporter	GFP
Gene	Otof
Gene Species	Mus musculus (Murine, House mouse)
Host Cell	Human embryonic kidney immortal cell line transformed with SV40 large T antigen (HEK293T)
Host Cell Species	Homo sapiens (human) cell line
Stability	This cell line is stable at least 10 passages.
Product Type	Mouse gene overexpression stable cell line
Applications	1) investigation of gene function 2) screening and validation of antibodies
Quality Control	1) Real-time qPCR analysis of gene mRNA overexpression level 2) GFP fluorescent detection under fluorescent microscopy 3) mycoplasma detection
Size Form	One vial of frozen cells, typically >1x10^6cells/vial
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Adherent

Gene Name	Otof otoferlin [ Mus musculus ]
Gene Symbol	Otof
Gene Description	otoferlin
Gene ID	83762
UniProt ID	E9PYR6
mRNA Refseq	NM_031875.2
Protein Refseq	NP_114081.2
Chromosome Location	5 B1; 5
Function	calcium ion binding; protein binding;

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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The Otof gene encodes otoferlin, a multi-C2 domain transmembrane protein crucial for hearing and balance in vertebrates. Otoferlin acts as a primary calcium ion sensor for exocytosis at the inner hair cell (IHC) synaptic band, responsible for the rapid release of neurotransmitters. Clinically, biallelic mutations in the Otof gene lead to DFNB9 congenital deafness. OTOF-targeted gene therapy using adeno-associated virus (AAV) vectors has shown promising results in clinical trials. Otoferlin is also involved in vesicle circulation and the maintenance of synaptic structure.

The stable mouse Otof cell line in the HEK293T context provides an ideal platform for evaluating the function of otoferlin and its gene therapy constructs. The HEK293T cell line is well-suited for expressing large proteins and producing high-titer AAV vectors. By stably overexpressing mouse Otof, this cell line provides a standardized environment for studying calcium ion sensing properties and their interactions with membrane transport proteins such as SNARE proteins. This product has invaluable value for screening compounds that regulate hair cell exocytosis and assessing the safety of AAV-OTOF.

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