Mouse Nr4a1 Stable Cell Line - MC3T3-E1

Name: Mouse Nr4a1 Stable Cell Line - MC3T3-E1
SKU: CSC-RO02545
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO02545

Host Cell : MC3T3-E1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO02545
Description	This cell line is engineered to overexpress Mouse Nr4a1 in MC3T3-E1 cell line. GFP reporter gene is also expressed in this cell line for cell tracking.
Product Type	Stable cell line constitutively expressing target gene
Target Gene	Nr4a1
Gene Species	Mouse
Host Cell	MC3T3-E1
Host Cell Species	Mus musculus (Mouse)
Applications	Gene function studies and others
Size	One vial of frozen cells, typically >1x10^6cells/vial
Stability	This cell line is stable at least 10 passages.
Quality Control	1) Detection of gene overexpression by Real-time qPCR ,or western blot, or flow cytometry, 2) Mycoplasma detection
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Target Gene

NR4A1

Background

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

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