Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RO01315
Host Cell : NIT-1 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RO01315 |
| Description | This cell line is engineered to stably express Mus musculus (Murine, House mouse) methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit (Mettl3) in Mouse insulinoma / pancreatic β-cell line (NIT-1). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells. |
| Product Type | Mouse gene overexpression stable cell line |
| Target Gene | Mettl3 |
| Gene Species | Mus musculus (Murine, House mouse) |
| Host Cell | NIT-1 |
| Host Cell Species | Mus musculus (Mouse) |
| Reporter | GFP |
| Applications |
1) investigation of gene function 2) screening and validation of antibodies |
| Size | One vial of frozen cells, typically >1x10^6cells/vial |
| Stability | This cell line is stable at least 10 passages. |
| Quality Control |
1) Real-time qPCR analysis of gene mRNA overexpression level 2) GFP fluorescent detection under fluorescent microscopy 3) mycoplasma detection |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Target Gene | METTL3 |
| Background | This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008] |
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This Mettl3 stable NIT-1 line has been instrumental in our study of RNA methylation in beta cells. The expression levels are consistent, and the cells are very easy to handle. It saved us months of work in stable cell line generation.
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