Mouse Gpx4 Stable Cell Line - RAW 264.7

The GPX4 gene encodes a unique selenoprotein, a key enzymatic defense mechanism against lipid peroxidation in biological membranes. GPX4 is a major regulator of ferroptosis (an iron-dependent, non-apoptotic cell death process). Clinically, GPX4 is crucial for brain health and the prevention of neurodegenerative diseases. In oncology, high GPX4 expression is closely associated with chemotherapy resistance and poor prognosis in various cancers because it enables tumor cells to resist oxidative stress. Targeting GPX4 has become a promising strategy for improving the chemosensitivity of drug-resistant malignancies.

A stable mouse GPX4 cell line in the RAW 264.7 background is the preferred model for studying ferroptosis in macrophage biology and innate immunity. RAW 264.7 cells are highly sensitive to lipid peroxidation and are often used to study the mechanisms of inflammatory cell death. By stably overexpressing mouse GPX4, this line provides a platform for evaluating how enhanced antioxidant capacity affects macrophage survival and the secretion of pro-inflammatory cytokines. This product has invaluable potential for screening GPX4 inhibitors or compounds that modulate the ferroptosis threshold.