Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RO01366
Host Cell : RAW264.7 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RO01366 |
| Description | This cell line is engineered to stably express Mus musculus (Murine, House mouse) eukaryotic translation elongation factor 1 alpha 1 (Eef1a1) in Mouse macrophage cell line (RAW 264.7). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells. |
| Product Type | Mouse gene overexpression stable cell line |
| Target Gene | Eef1a1 |
| Gene Species | Mus musculus (Murine, House mouse) |
| Host Cell | RAW264.7 |
| Host Cell Species | Mus musculus (Mouse) |
| Reporter | GFP |
| Applications |
1) investigation of gene function 2) screening and validation of antibodies |
| Size | One vial of frozen cells, typically >1x10^6cells/vial |
| Stability | This cell line is stable at least 10 passages. |
| Quality Control |
1) Real-time qPCR analysis of gene mRNA overexpression level 2) GFP fluorescent detection under fluorescent microscopy 3) mycoplasma detection |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Target Gene | EEF1A1 |
| Background | This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008] |
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The Mouse Eef1a1 stable line from Creative Biogene has been a perfect addition to our translational research. The cell morphology is excellent, and the transgene expression is robust. It has provided the high level of reproducibility we needed for our protein synthesis assays in macrophages.
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