ISRE-Luc/EF1α-mCherry Reporter Cell Line - U87MG

ISRE-Luc/EF1α-mCherry Reporter Cell Line - U87MG is a genetically engineered human glioblastoma cell line derived from the parental U87MG model. The cells contain a luciferase reporter gene controlled by an interferon-stimulated response element (ISRE), enabling quantitative monitoring of signaling pathways activated by type I and type III interferons and related innate immune stimuli. In addition, mCherry is constitutively expressed under the EF1α promoter, providing a stable fluorescent marker for identifying, tracking, and normalizing viable reporter cells during routine culture and experimental procedures. The dual-reporter design combines sensitive luminescence-based pathway measurement with convenient fluorescence-based cell visualization.

This reporter cell line is primarily intended for evaluating ISRE pathway activation and inhibition in a high-throughput or mechanistic research setting. It can be used to assess the biological activity of interferons, interferon-inducing agents, pattern-recognition receptor agonists, antiviral compounds, and other immunomodulatory molecules. The luciferase signal provides a convenient quantitative readout for dose-response analysis, pathway kinetics, compound ranking, and characterization of agonist or antagonist potency. Constitutive EF1α-mCherry expression supports cell counting, imaging, culture-quality monitoring, and assay normalization, helping distinguish changes in pathway activity from variations in cell number or viability. The cells may also be incorporated into co-culture experiments, gene-function studies, and investigations of tumor-associated innate immune responses. By combining the U87MG glioblastoma background with a sensitive ISRE reporter, this product can support drug discovery, antiviral research, cancer immunology, and studies of interferon-related signaling.