Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CC-692
| Cat. No. | CC-692 |
| Description | A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption. |
| Gene Abbr | Il7r |
| Gene Name | Il7r interleukin 7 receptor [ Mus musculus ] |
| Species | Mouse |
| EnsemblID | ENSMUSG00000003882 |
| NCBIGeneID | 16197 |
| Uni ProtID | P16872 |
| Features |
|
| Applications | This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout. |
| Reactions | 5–10 reactions per target gene |
| Kit Components |
2–3 chemically synthesized sgRNAs (200pmol each) 3 PCR/Sequencing primers (500pmol each) LM cell lysate (500µL) Cas9 protein (12µg) LM RNP transfection reagent (50µL) |
| Storage | Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles. |
| Gene Name | Il7r interleukin 7 receptor [ Mus musculus ] |
| Gene Symbol | Il7r |
| Synonyms | CD127; IL-7Ralpha |
| Gene Description | interleukin 7 receptor |
| GeneID | 16197 |
| Uni ProtID | P16872 |
| mRNA Refseq | NM_008372.4 |
| Protein Refseq | NP_032398.3 |
| Chromosome Location | 15 A1; 15 4.6 cM |
| Function | cytokine receptor activity; |
| Pathway | Cytokine Signaling in Immune system, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; IL-7 Signaling Pathway, organism-specific biosystem; Immune System, organism-specific biosystem; |
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