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CC-692

IL7R Easy KO Kit (Mouse)

For research use only. Not intended for any clinical use.

Cat. No. :   CC-692

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Product Information

Gene Information

Cat. No. CC-692
Description A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Gene Abbr Il7r
Gene Name Il7r interleukin 7 receptor [ Mus musculus ]
Species Mouse
EnsemblID ENSMUSG00000003882
NCBIGeneID 16197
Uni ProtID P16872
Features
  • All-in-One workflow from gene editing to knockout validation for users with no prior experience.
  • Pre-validated sgRNAs and primers for rapid setup.
  • Streamlined experiment handling.
  • CRISPR RNP method ensures precise and efficient gene knockout.
Applications This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Reactions 5–10 reactions per target gene
Kit Components 2–3 chemically synthesized sgRNAs (200pmol each)
3 PCR/Sequencing primers (500pmol each)
LM cell lysate (500µL)
Cas9 protein (12µg)
LM RNP transfection reagent (50µL)
Storage Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.
Gene Name Il7r interleukin 7 receptor [ Mus musculus ]
Gene Symbol Il7r
Synonyms CD127; IL-7Ralpha
Gene Description interleukin 7 receptor
GeneID 16197
Uni ProtID P16872
mRNA Refseq NM_008372.4
Protein Refseq NP_032398.3
Chromosome Location 15 A1; 15 4.6 cM
Function cytokine receptor activity;
Pathway Cytokine Signaling in Immune system, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; IL-7 Signaling Pathway, organism-specific biosystem; Immune System, organism-specific biosystem;
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